Visualization of Nucleic Acids

Front Cover
CRC Press, 1995 M03 30 - 384 pages
This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.
 

Contents

THE CLASSIC METHODS OF NUCLEIC ACID VISUALIZATION
3
Chapter
5
References
19
Imaging Nucleic Acids with Scanning Probe Microscopes
21
be divided into classical morphological staining histochemical methods and the use
33
Chapter 3
35
DNA in Its Aqueous Environment
57
Chapter 6
95
Chapter 10
169
Chapter 11
185
Chapter 12
201
Chapter 13
213
Chapter 14
229
Chapter 15
259
Chapter 16
277
FIXATION OF NUCLEIC ACIDS
316

acids on paraffin sections Where the technique can be used with smears such is indicated
96
Chapter 7
111
Chapter 8
137
Chapter 9
155
Chapter 17
323
Index
357
Copyright

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