Visualization of Nucleic AcidsCRC Press, 1995 M03 30 - 384 pages This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR. |
Contents
THE CLASSIC METHODS OF NUCLEIC ACID VISUALIZATION | 3 |
Chapter | 5 |
References | 19 |
Imaging Nucleic Acids with Scanning Probe Microscopes | 21 |
be divided into classical morphological staining histochemical methods and the use | 33 |
Chapter 3 | 35 |
DNA in Its Aqueous Environment | 57 |
Chapter 6 | 95 |
Chapter 10 | 169 |
Chapter 11 | 185 |
Chapter 12 | 201 |
Chapter 13 | 213 |
Chapter 14 | 229 |
Chapter 15 | 259 |
Chapter 16 | 277 |
FIXATION OF NUCLEIC ACIDS | 316 |
acids on paraffin sections Where the technique can be used with smears such is indicated | 96 |
Chapter 7 | 111 |
Chapter 8 | 137 |
Chapter 9 | 155 |
Chapter 17 | 323 |
Index | 357 |
Common terms and phrases
Acad adenovirus ammine analysis antibody antigen autoradiography binding Biochem biological biotin biotinylated buffer capsid Cell Biol cellular chromatin chromosome colloidal gold complex cryoelectron microscopy Cytochem cytometry cytoplasm denaturation detection distilled water DNA molecules DNA probes DNase electron microscopy embedding Figure film fixation flow cytometry fluorescence gene genomes gold particles grids herpes simplex virus high resolution Histochem hybridization hydrolysis incubation infected cells interactions labeling localization Lowicryl mapping membrane method Microsc molecular monoclonal mRNA Natl nonradioactive nuclear nuclease-gold nucleolus nucleoprotein nucleosomes nucleotides observed obtained oligonucleotide osmium ammine Pathol polymerase chain reaction Proc procedure protein Protocol Puvion-Dutilleul radioactive replication revealed Révet ribosomal RNA polymerase RNase RNase-gold samples scanning sensitivity sequences single-stranded DNA situ hybridization slides solution specific specimens staining structures technique temperature thin sections transcription ultrastructural ultrathin sections uranyl acetate viral DNA viral genomes Virol viruses visualization vitro