Plant Tissue Culture Manual - Supplement 7: Fundamentals and ApplicationsK. Lindsey Springer Science & Business Media, 2013 M11 11 - 1187 pages Plant tissue culture has a long history, dating back to the work of Gottlieb Haberlandt and others at the end of the 19th century, but the associated concepts and techniques have reached a level of usefulness and application which has never been greater. The technical innovations have given new insights into fundamental aspects of plant differentiation and development, and have paved the way to the identification of strategies for the genetic manipula tion of plants. It is the aim of this manual to deliver a broad range of these techniques in a form which is accessible to students and research scientists of diverse backgrounds, including those with little or no previous experience. The themes of the manual aim to reflect those research areas which have been advanced by tissue culture technology. As was the case for the sister volume Plant Molecular Biology Manual, the objective has been from the start to produce a manual which is at home on the laboratory bench. The plastic-covered, ring-bound format has proved to be most popular and is retained here. Equally, the emphasis has been on producing a collection of detailed step-by-step protocols, each supplemented with an introductory text and practical footnotes, to provide the next best thing to a supervisor at one's shoulder. |
Contents
9 | |
24 | |
24 | |
Shoot cultures and root cultures of tobacco | 24 |
Somatic embryogenesis in orchardgrass | 24 |
Arabidopsis regeneration and transformation leaf cotyledon explant | 24 |
Arabidopsis protoplast transformation and regeneration | 24 |
Arabidopsis regeneration and transformation root explant system | 26 |
PROPAGATION CONSERVATION OF GERMPLASM | 47 |
Clonal propagation of palms | 55 |
Clonal propagation of conifers | 65 |
Clonal propagation of Citrus | 65 |
Clonal propagation of eucalypts | 47 |
the example of embryogenic | 65 |
DIRECT GENE TRANSFER PROTOPLAST FUSION | 83 |
Cybrid production and selection | 99 |
Somatic embryogenesis in carrot | 26 |
microdroplets and singlecell nurse cultures | 33 |
Tobacco protoplast isolation culture and regeneration | 40 |
TISSUE CULTURE TRANSFORMATION OF CROP | 40 |
Transformation and regeneration of rice protoplasts | 40 |
Regeneration and transformation of potato by Agrobacterium tumefaciens | 40 |
Transformation of tomato with Agrobacterium tumefaciens | 40 |
Regeneration and transformation of sugarbeet by Agrobacterium | 40 |
Regeneration and transformation of apple Malus pumila Mill | 40 |
Transformation and regeneration of maize protoplasts | 40 |
Regeneration and transformation of barley protoplasts | 40 |
Agrobacteriummediated transformation of potato stem and tuber tissue | 40 |
Production of fertile transgenic wheat by microprojectile bombardment | 40 |
Transient gene expression and stable genetic transformation into conifer | 40 |
Fluorescenceactivated analysis and sorting of protoplasts and somatic | 79 |
RFLP analysis of organellar genomes in somatic hybrids | 94 |
Isolation and uptake of plant nuclei | 102 |
Radioactive | 119 |
Chemical fusion of protoplasts | 9 |
REPRODUCTIVE TISSUES | 17 |
MUTANT SELECTION | 90 |
SECONDARY METABOLITES | 99 |
TISSUE CULTURE TECHNIQUES | 99 |
Establishment of photoautotrophic cell cultures | 99 |
Zinnia mesophyll culture system to study xylogenesis | 99 |
181 | |
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Plant Tissue Culture Manual - Supplement 7: Fundamentals and Applications K. Lindsey No preview available - 2014 |
Common terms and phrases
acid agar agarose Agrobacterium alkaloids anthocyanin antibiotic Arabidopsis thaliana assay autoclaving auxin bombardment buds buffer CaCl2 callus carrot cell cultures cell suspension cultures centrifuge chromosomes concentration containing culture medium density distilled water efficiency electroporation embryogenesis embryogenic embryogenic cell embryogenic suspension embryos endosperm enzyme ethanol filter flask frequency fusion gene transfer genotype germination growth H₂O Incubate induction isolated kanamycin leaf liquid maize mannitol marker method mg/l mg/ml microcapillary microculture chamber molecular Murashige obtained Petri dish pipette Plant Cell Plant Cell Rep plant regeneration Plant Tissue Culture plantlets plasmid plates pollen procedure protocol protoplasts remove resuspend roseus seedlings seeds selection shoot cultures sieve single cell somatic embryogenesis somatic embryos somatic hybrids species stage Steps sterile stock solution subculture sucrose supernatant Theor Appl Genet Tissue Culture Manual tobacco transformation transgenic tubes Vasil vitamins vitro volume Wash weeks µg/ml