Computer Operation for Microscope Photometry
CRC Press, 1997 M11 25 - 256 pages
Suitable for both microscopists seeking computer skills and PC enthusiasts interested in light microscopy, this interdisciplinary text explores the capabilities of the computer-assisted light microscope. Written in clear, simple language, the book explains how computer technology expands the usefulness of the light microscope in spectrophotometry, fluorometry, polarimetry, spatial scanning, and related fields.
Beginning with the basic features of light microscopy and personal computer interfacing, the text explains how to make photometric measurements and covers spectrophotometry, stepper motors, and server motors. Polarized light and video image analysis complete this introduction to the field. While software examples are provided to illustrate specific techniques, most operations are described as generalized algorithms that can be adapted to any appropriate high-level language, and used with almost any configuration of the microscope. The book suggests new experiments to inspire further study. Promising new areas of interest, such as the use of fluorescence and polarization, are also included.
Computers have radically changed the field of light microscopy in recent decades. Computer Operations for Microscope Photometry helps you master the new techniques.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Shutters and Apertures
Monochromators and Spectrophotometry
Mechanical Stages and Scanning
Other editions - View all
absorbance acceptance adjusted allows angle aperture become birefringence called caused Chapter closed compensator created device direction dynamic range effect emission emission spectrum enter example excitation experiment fiberoptic field Figure filter fluorescence gain glass illumination increased intensity lamp length light light guide maximum measurements method microscope monochromator motor mounted move muscle normal objective obtained operator optical axis optical fiber OUTPUT passing path peak phase photometer pixel polarized position possible PRINT problem produce reach reduced reflectance refractive index relative response rotation sample scanner scanning separate shown shows shutter signal simple specimen spectra spectrum stage standard step structure surface Teflon temperature tilting tissue transmittance usually voltage WAIT wavelength window Zeiss