Advanced Techniques in Chromosome ResearchKenneth W. Adolph CRC Press, 1991 M03 28 - 472 pages Contributors detail up-to-date guidelines for using molecular techniques, cytogenetic and linkage analysis, and cellular methods, emphasizing human cells and medically relevant research. They present results of recent applications of techniques and step-by-step protocols for cloning large DNA molecu |
Contents
Molecular Techniques in Genetic and Chromosomal Diagnosis | 3 |
Chapter | 13 |
Cloning and Analysis of Large DNA Molecules | 33 |
Molecular Delineation of a Chromosomal SubBand Commonly | 73 |
Chapter 4 | 95 |
In Situ Hybridization of Nonradioactive Oligonucleotide | 117 |
Chapter 6 | 135 |
PulsedField Gel Electrophoresis and Its Application | 155 |
Molecular Linkage Genetics of the Mouse | 249 |
Chapter 12 | 269 |
Strategies for Locus Ordering | 297 |
Cellular Techniques | 331 |
Chapter 15 | 349 |
Mammalian Cells for Testing Genotoxicity of Chemical Mutagens | 391 |
Primary Culture of Solid Lung Tumors for Chromosomal Analysis | 411 |
Chapter 18 | 427 |
Common terms and phrases
Acad agarose allele amplification analysis antibodies antigens band Biol BrdU breakpoint buffer Cancer cell line centromere cerevisiae chro chromatid chromo chromosome 11 Colcemid colchicine containing culture cytogenetic detection digestion DNA fragments DNA probes DNA sequences duplicated EDTA electric field embryos field gel Figure fragile sites gel electrophoresis gene mapping Genet human chromosomes incubated inserts karyotype kinetochores laboratory leukemia libraries ligation linkage maps loci locus LOD scores lymphocyte lymphoma malignant mammalian markers medium melanoma metaphase metaphase chromosomes method microtubule mitotic molecular mosomes mutations Natl normal Nucleic Acids Res oligonucleotide oncogene PFGE physical map polymorphic Proc protein pulsed field rearrangements recombination fractions region restriction enzyme restriction fragment RFLP sample DNA segments separation Southern blot specific spheroplasts staining structure studies techniques temperature tion transgenic mice translocation tube tumor vector Y chromosome YAC clones yeast artificial chromosomes yeast chromosomes