Cellular Electron MicroscopyJ. Richard McIntosh Elsevier, 2011 M09 2 - 880 pages Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.
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Contents
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Imaging Fixed Cells in Three Dimensions | 121 |
Imaging FrozenHydrated Cells and Cell Parts | 369 |
Localizing Macromolecules in Cells | 489 |
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Common terms and phrases
3D reconstruction actin actin filaments algorithm alignment analysis antibody antigen Archaea average basal body Baumeister biological buVer CCDB Cell Biol Cell Biology cellular Chapter clathrin complex contrast correlation cryo-electron cryo-electron microscopy cryo-EM cryosections cytoplasmic cytoskeleton database density detector diVerent diVusion diYcult domain dose Dubochet electron microscopy electron tomography embedding embryos eVect eYciency filter fixation fluorescent freeze-substitution frozen frozen-hydrated function glutaraldehyde gold particles Golgi grid helical high-pressure freezing immunolabeling kinesin kinetochore labeling macromolecules Mastronarde McIntosh membrane methods Microsc microtubules mitotic mitotic spindle molecular molecules muscle noise O’Toole organelles orientation pixel protein receptor resin resolution ribosomes sample Scale bar ¼ scintillator segmentation serial sections single-particle slice spatial specimen spindle SSNR stained Struct structure studies surface suYcient synapses techniques temperature template thick Three-dimensional tilt series tissue tomograms tomographic reconstruction Ultramicroscopy ultrastructure uranyl acetate vesicles virus visualized vitrified volume yeast
Popular passages
Page xxvi - Pardue, ML (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
Page 53 - The response of biological macromolecules and supramolecular structures to the physics of specimen cryopreparation. In Cryotechniques in Biological Electron Microscopy (RA Steinbrecht and K.
Page xxvi - Mclntosh R, Nicastro D and Mastronarde D (2005) New views of cells in 3D: An introduction to electron tomography. Trends Cell Biol...
Page 56 - Brenner S., 1975. Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabduis elegans. J. Comp.Neurol. 160,313-337. Ware RW, Clark D., Crossland K., and Russell RL, 1975. The nerve ring of the nematode Caenorhabditis elegans: Sensory input and motor output I.