In Situ Hybridization in Neurobiology: Advances in Methodology

Front Cover
James H. Eberwine, Karen L. Valentino, Jack D. Barchas
Oxford University Press, 1994 - 199 pages
In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situ hybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridization technology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. It discusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
 

Contents

Methodological Considerations in the Utilization of In Situ
3
Combining Nonisotopic In Situ Hybridization Histochemistry with Steroid
24
Development of Techniques to Combine Isotopic and Nonisotopic In Situ
43
Enzyme Histochemical Detection of Neuronal mRNA
63
Characterization of the Receptor Complement of Individual Neurons
78
Assessment of Peptide Secretion and Gene Expression Within Individual
96
Quantitative MicroIn Situ Hybridization
108
Analysis of Transcription Levels Using Intervening Sequence In Situ
124
A Powerful New Methodology
143
Methodology and Multifaceted Applications
157
Index
193
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