Nonradioactive Analysis of BiomoleculesChristoph Kessler Springer Science & Business Media, 2000 M03 11 - 748 pages New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct the appropriate procedures which fulfill their particular requirements. |
Contents
Introduction | 1 |
Chapter | 7 |
Chapter 39 | 24 |
Part A Standard Nonradioactive Labeling Systems | 50 |
Chapter | 56 |
Chapter 5 | 94 |
Labeling of Proteins During In Vitro Translation | 137 |
Chapter 8 | 155 |
Chapter 48 | 526 |
New Tools for Multicolor Chromosome Analysis | 539 |
Chapter 49 | 557 |
Chapter 50 | 573 |
Quantitative Formats | 594 |
Chapter 54 | 617 |
PCRCoupled Activation of Intercalating Dyes | 635 |
Sequencing Formats | 648 |
5BrdU AAF Gold Labeling | 168 |
Chapter 11 | 193 |
Chapter 13 | 216 |
Chapter 15 | 240 |
Chapter 17 | 253 |
Chapter 20 | 271 |
Chapter 23 | 289 |
Fluorescence Systems | 327 |
Chapter 28 | 343 |
Strand Displacement Amplification SDA Target Amplification | 356 |
Isothermal Amplification of | 374 |
Signal Amplification | 388 |
Chapter 35 | 407 |
Chapter 37 | 437 |
PNA PreGel Hybridization An Alternative to Southern Blotting | 464 |
In Situ Formats | 478 |
Chapter 44 | 494 |
Chapter 46 | 516 |
Chapter 59 | 675 |
Chemiluminescent Detection | 689 |
Array Formats | 713 |
Use of Oligonucleotide Arrays for Gene Expression Monitoring | 738 |
Streptavidin System | 6 |
An Integrated Software Development Environment | 12 |
H OSWALD CH | 21 |
Configuration Management in an Open Environment | 35 |
Software Management Constraints and Action Triggering in | 44 |
A Logic | 55 |
Delta Technique and StringtoString Correction | 64 |
ASDL An ObjectOriented Specification Language for Syntax | 71 |
A Hybrid Interpreter in a Software Development Environment | 80 |
G ENGLES A SCHURR | 89 |
Labeling and Detection of Nucleic Acids | 111 |
S BEER R WELLAND I SOMMERVILLE UK | 272 |
Labeling of Biomolecules with Fluorophores | 284 |
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Common terms and phrases
alkaline phosphatase amplification Anal Biochem analysis antibody applications assay base binding biotin biotin-labeled biotinylated blocking blot buffer cells Centrifuge chemiluminescent chemiluminescent detection chromosome clones concentration configuration conjugate cycle database denaturation detection of nucleic detection systems DIG-labeled digoxigenin diluted DNA probes DNA sequencing EDTA electrophoresis environment enzymatic enzyme ethanol filter fluorescence formamide fragments gene genetic genomic hapten Hoeltke hybridization probes hybridization solution immunoassay Incubate Kessler Kricka labeled probe labeling and detection membrane method MgCl2 molecules mRNA NaCl nitrocellulose Nonradioactive Labeling Northern blots nucleic acid hybridization Nucleic Acids Res nucleotides oligonucleotide probes PCR products phosphate polymerase chain reaction primer procedure proteins protocol reaction reagents Roche Molecular Biochemicals room temperature sample sensitivity signal situ hybridization slides specific staining Subprotocol substrate synthesis target template tion transcription transfer translation Tris-HCl tube vitro Wash Wilchek µg/ml