Receptor Binding Techniques

35S]GTPYS binding 5-HT3 receptor acid affinity constant agonist aliquot analysis angiotensin antagonist approx autoradiography benzodiazepine binding assays binding sites binding studies Biochem Biol buffer cells centrifugation complex cover slips curve density DEPC-treated detection detergent dH₂O digitonin diluted dissociation EDTA emulsion enzyme equations equilibrium ethanol film filter functional G protein G protein-coupled receptors GABA guanine homogenate hybridization ice-cold imidazoline incubation inhibition inhibitors interaction kinetics labeled ligand ligand binding measured membrane preparation method microcentrifuge microcentrifuge tubes molecular muscarinic acetylcholine receptors muscarinic receptors nonspecific binding Note ondansetron parameters pellet Pharmacol pipet probe procedures Protocols radioactivity radiolabeled radioligand binding recep receptor binding receptor preparation receptor subtypes remove riboprobe room temperature samples scan scintillation Sigma slides solubilized receptor solution specific activity specific binding stored at 4°C Subheading sucrose supernatant tion tracer ligand tubes unlabeled vials wash