In Situ Hybridisation: Application to Developmental Biology and Medicine

Front Cover
N. Harris, D. G. Wilkinson, Laboratory of Developmental Neurobiology D G Wilkinson
Cambridge University Press, 1990 M08 31 - 288 pages
Advances in our understanding of biological mechanisms have frequently been associated with the development of new techniques. In situ hybridization is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, where it is giving new insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of this technique and will help the reader review and be guided through the various options and variations of the technique.
 

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This Book really contains the information the most needed for In-Situ Hybridization; It is really recommended to read it while you are doing the protocol at your lab.

Contents

Use of haptenised nucleic acid probes in fluorescent in situ
33
Contributions of the spatial analysis of gene expression to the study
69
Advantages and limitations of in situ hybridisation as exemplified
97
The use of in situ hybridisation to study the localisation of maternal
115
In situ hybridisation in the analysis of genes with potential roles
131
McFadden
144
Localisation of expression of male flowerspecific genes from maize
157
Tissue preparation techniques for in situ hybridisation studies
175
Grindley
186
Investigation of gene expression during plant gametogenesis by
189
Sexing the human conceptus by in situ hybridisation
205
Nonisotopic in situ hybridisation in human pathology
241
The demonstration of viral DNA in human tissues by in situ
271
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