Immunocytochemical Methods and ProtocolsLorette C. Javois Springer Science & Business Media, 2008 M02 2 - 465 pages Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization. |
Contents
PART II TISSUE PREPARATION FOR LIGHT MICROSCOPIC ANALYSES | 43 |
LIGHT MICROSCOPIC DETECTION SYSTEMS | 95 |
FLUORESCENCE ACTIVATED CELL SORTER FACS ANALYSES | 247 |
COLLOIDAL GOLD DETECTION SYSTEMS FOR ELECTRON MICROSCOPIC ANALYSES | 317 |
APPLICATIONS FOR NUCLEIC ACID LOCALIZATION BY IN SITU HYBRIDIZATION | 355 |
SPECIAL APPLICATIONS FOR THE CLINICAL LABORATORY | 403 |
Index | 459 |
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Common terms and phrases
acid anti antigen assay avidin binding biotin biotinylated blocking solution buffer cell suspension cellular centrifuge Chapter chromatography chromogen chromosome colloidal gold column concentration confocal confocal microscopy conjugated containing coverslip Cytochem deionized glass-distilled water detection diluted dish endogenous enzyme ethanol filter fixation fixative flow cytometry fluorescein fluorescence fluorochrome formaldehyde glutaraldehyde Histochem human Humana Press Inc hybridization Immunocytochemical Methods immunocytochemistry immunofluorescence immunoglobulin immunohistochemical Immunol immunostaining incubation L. C. Javois labeling laboratory laser membrane Methods and Protocols Methods in Molecular microscope Molecular Biology molecules monoclonal antibody neutrophils nonspecific Note paraffin paraffin-embedded particles Pathol PBSG pellet permeabilization peroxidase phosphate pipet preparation primary antibody probe procedure protein reaction reactive reagent remove resuspend rhodamine Rinse room temperature sample secondary antibody serum slides sodium specific specimen staining step streptavidin Subheading substrate surface technique tion tissue sections Totowa tubes tumors vial wash µg/mL